Journal: PLoS ONE

Article Title: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls

doi: 10.1371/journal.pone.0108336

Figure Lengend Snippet: (a) Quantification of ABCC6 mRNA expression in human dermal fibroblasts from healthy controls (n = 5; white) and PXE patients (n = 6; black). Effect of siRNA-mediated knockdown on ABCC6 gene expression: fibroblasts transfected with a scramble siRNA-negative control (siNK, n = 3; white); ABCC6-specific siRNA-treated cells (siABCC6, n = 3; black). Expression levels are normalized to reference gene expressions (ACTB, GAPDH, ß2M). Data are presented in arbitrary units as means with corresponding standard error. Differences between controls and PXE fibroblasts, as well as between siRNA-transfected cells, were analyzed using unpaired t-test with Welch's correction. (b) Western Blot analysis of ABCC6 in fibroblasts of healthy controls, PXE patients and siRNA- treated cells. Western blot analysis was performed with pooled protein samples from each group, with GAPDH as normalization control. HEK-293 cell lysates were used as an additional blotting control. (c) Representative Western Blot for fibroblasts of healthy controls, PXE patients and (d) siRNA- treated cells.

Article Snippet: Dermal fibroblasts from six healthy controls (NHDF) were purchased from Promocell (Heidelberg, Germany), Genlantis (San Diego, USA), Cambrex (Walkersville, USA) and Coriell (New Jersey, USA).

Techniques: Expressing, Knockdown, Gene Expression, Transfection, Negative Control, Western Blot, Control

Journal: PLoS ONE

Article Title: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls

doi: 10.1371/journal.pone.0108336

Figure Lengend Snippet: Summary of metabolic findings in PXE human dermal fibroblasts compared to healthy controls.

Article Snippet: Dermal fibroblasts from six healthy controls (NHDF) were purchased from Promocell (Heidelberg, Germany), Genlantis (San Diego, USA), Cambrex (Walkersville, USA) and Coriell (New Jersey, USA).

Techniques:

Journal: BMJ Open Ophthalmology

Article Title: Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells

doi: 10.1136/bmjophth-2018-000217

Figure Lengend Snippet: Representative fluorescent microscopic images of immunostained cells with anti-pan-keratin antibody and its isotype control antibody (mouse IgG1). (A, B) Cells from human corneal surface origin (peripheral portion) and normal human dermal fibroblasts immunostained with anti-pan-keratin antibody, respectively. (C, D) Cells from human corneal surface origin (peripheral portion) and normal human dermal fibroblasts immunostained with an isotype control antibody (mouse IgG1), respectively. Scale bar=100 µm.

Article Snippet: Normal human dermal fibroblasts (NHDF, DS Pharma Biomedical, Osaka, Japan) were used as a negative control.

Techniques: Control

Journal: International Journal of Cosmetic Science

Article Title: Multidirectional activity of bakuchiol against cellular mechanisms of facial ageing ‐ Experimental evidence for a holistic treatment approach

doi: 10.1111/ics.12784

Figure Lengend Snippet: Antioxidative and anti‐inflammatory capacity of bakuchiol and retinol. (a) Antioxidative efficacy of bakuchiol (100 μM) or retinol (100 μM) compared to the high standard trolox (25 μM) and the solvent control determined by a DPPH antioxidant assay through absorbance measurement at 524 nm after 10, 30 and 60 min. N = 12. (b) Antioxidative power expressed in antioxidative units of bakuchiol, retinol or the high standard vitamin C (vit. C) determined using electron spin resonance spectroscopy. (c) ELISA‐based measurement of prostaglandin E2 (PGE2) levels in unstressed human dermal fibroblasts (HDFs), LPS‐stressed control HDFs, and in LPS‐stressed HDFs treated with the high standard diclofenac (25 ng/mL), bakuchiol or retinol (both applied at 1.25, 2.5, 5, 10 μM) for 24 h. N = 12. (d) Macrophage migration inhibitory factor (MIF) protein levels in unstressed HDFs, in HDFs stressed by a DPBS incubation and in stressed HDFs treated with bakuchiol or retinol (both applied at 1 and 10 μM) for 48 h determined by ELISA. N = 10. Results are depicted as mean ± SD. Statistics were performed by RM‐ANOVA with post‐hoc pairwise comparison based on Blom‐transformed ranks for Figure or by a pairwise Wilcoxon signed rank test for Figure . Significant differences are marked with an asterisk (** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001) [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Human dermal fibroblasts (HDFs) from multiple donors were obtained from Tissue Solutions Ltd (Glasgow, UK), Lonza (Basel, Switzerland), tebu‐bio (Heerhugowaard, Netherlands) or isolated from full‐thickness skin explants purchased from Alphenyx (Marseille, France) as described before [ ].

Techniques: Solvent, Control, Antioxidant Assay, Electron Paramagnetic Resonance, Spectroscopy, Enzyme-linked Immunosorbent Assay, Migration, Incubation, Comparison, Transformation Assay

Journal: International Journal of Cosmetic Science

Article Title: Multidirectional activity of bakuchiol against cellular mechanisms of facial ageing ‐ Experimental evidence for a holistic treatment approach

doi: 10.1111/ics.12784

Figure Lengend Snippet: Effects of bakuchiol and retinol on cell activity and major ECM components. (a) Fibroblast growth factor 7 (FGF7) protein levels in control HDFs and in HDFs treated with 10 μM bakuchiol or retinol for 24 h. N = 13. (b) Quantification of WST‐1 metabolization in control HDFs and in HDFs treated with 10% triton‐X, bakuchiol or retinol (both applied at 1 and 10 μM) for 72 h. Difference in absorbance at 620 and 450 nm is depicted. N = 12. (c) Collagen, type VII, alpha 1 (COL7A1) protein levels in control HDFs and in HDFs treated with the high standard TGF‐β (10 ng/mL) and sodium ascorbate (SA; 11 μg/mL), as well as with bakuchiol or retinol (4 h: 1 and 10 μM, extended incubation: 10 μM for both test substances) for 4 h or for an extended incubation time (72 h or 96 h depending on cell confluence). N = 10 for 4 h, n = 11 for extended incubation. (d) Collagen, type I, alpha 1 (COL1A1) protein levels in control HDFs and in HDFs treated with the high standard TGF‐β and SA as aforementioned, bakuchiol or retinol (both applied at 1 and 10 μM) for 4 h. N = 11. (e) Fibronectin (FN) protein levels in control HDFs and in HDFs treated with 10 μM bakuchiol or retinol for 24 h. N = 11. All protein levels were determined by ELISA. Results are depicted as mean ± SD. Statistics were performed by RM‐ANOVA with post‐hoc pairwise comparison for Figure or by a pairwise Wilcoxon signed rank test for Figure . Significant differences are marked with an asterisk (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001) [Colour figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Human dermal fibroblasts (HDFs) from multiple donors were obtained from Tissue Solutions Ltd (Glasgow, UK), Lonza (Basel, Switzerland), tebu‐bio (Heerhugowaard, Netherlands) or isolated from full‐thickness skin explants purchased from Alphenyx (Marseille, France) as described before [ ].

Techniques: Activity Assay, Control, Incubation, Enzyme-linked Immunosorbent Assay, Comparison